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rabbit polyclonal leptin antibody against rat leptin  (BioVendor Instruments)


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    BioVendor Instruments rabbit polyclonal leptin antibody against rat leptin
    Figure 8 | Adipokine treatment. Effects of <t>leptin</t> (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.
    Rabbit Polyclonal Leptin Antibody Against Rat Leptin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+leptin+antibody+against+rat+leptin/pm20336056-133-17-24?v=BioVendor+Instruments
    Average 90 stars, based on 2 article reviews
    rabbit polyclonal leptin antibody against rat leptin - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture."

    Article Title: Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

    Journal: Kidney international

    doi: 10.1038/ki.2010.68

    Figure 8 | Adipokine treatment. Effects of leptin (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.
    Figure Legend Snippet: Figure 8 | Adipokine treatment. Effects of leptin (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.

    Techniques Used: Control, Concentration Assay

    Figure 9 | Adiponectin and leptin production of adipose tissue fragments (ATFs) in the absence or presence of Madin–Darby canine kidney (MDCK) cells in the supernatants of outer and inner dishes at 7 days in culture. In the cultures of ATFs alone, adiponectin and leptin are detected. Their concentration is not significantly different between the outer and inner dishes (P40.05). In the supernatants of outer dishes, adiponectin concentrations in the cocultures of ATFs and MDCK cells are significantly higher than those in the cultures of ATFs alone (Po0.05), whereas leptin concentrations are not significantly different between cultures of ATFs with and without MDCK cells (P40.05). In cocultures of ATFs and MDCK cells, adiponectin (Po0.01) and leptin (Po0.05) concentrations in the inner dishes were significantly lower than those in the outer dishes.
    Figure Legend Snippet: Figure 9 | Adiponectin and leptin production of adipose tissue fragments (ATFs) in the absence or presence of Madin–Darby canine kidney (MDCK) cells in the supernatants of outer and inner dishes at 7 days in culture. In the cultures of ATFs alone, adiponectin and leptin are detected. Their concentration is not significantly different between the outer and inner dishes (P40.05). In the supernatants of outer dishes, adiponectin concentrations in the cocultures of ATFs and MDCK cells are significantly higher than those in the cultures of ATFs alone (Po0.05), whereas leptin concentrations are not significantly different between cultures of ATFs with and without MDCK cells (P40.05). In cocultures of ATFs and MDCK cells, adiponectin (Po0.01) and leptin (Po0.05) concentrations in the inner dishes were significantly lower than those in the outer dishes.

    Techniques Used: Concentration Assay



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    rabbit polyclonal leptin antibody against rat leptin  (BioVendor Instruments)
    90
    BioVendor Instruments rabbit polyclonal leptin antibody against rat leptin
    Figure 8 | Adipokine treatment. Effects of <t>leptin</t> (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.
    Rabbit Polyclonal Leptin Antibody Against Rat Leptin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+leptin+antibody+against+rat+leptin/pm20336056-133-17-24?v=BioVendor+Instruments
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal leptin antibody against rat leptin - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 8 | Adipokine treatment. Effects of leptin (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.

    Journal: Kidney international

    Article Title: Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

    doi: 10.1038/ki.2010.68

    Figure Lengend Snippet: Figure 8 | Adipokine treatment. Effects of leptin (b–e), its antibody (insets in b–e), and combination of leptin and adiponection (f) on morphology of Madin–Darby canine kidney (MDCK) cells at 7 days in culture. (a) (Control), MDCK cells without leptin and adiponectin treatment show a flat shape with cytoplasm and flattened nuclei. (b and c) 20 (b) or 50 ng/ml (c) leptin does not affect the morphology of MDCK cells. (d and e) 100 (d) or 1000 ng/ml (e) leptin allows MDCK cells to develop a tall and large columnar shape, with clear cytoplasm and vertically and basally situated nuclei. The leptin-induced morphology is similar to the adipose tissue fragment (ATF)- induced morphology of MDCK cells. Leptin antibody (L-ab, 25 mg/ml) abolishes the MDCK cell morphology induced by 100 (inset in d) or 1000 ng/ml leptin (inset in e), although the antibody does not affect the morphology of cells at the concentration of 20 (inset in b) or 50 ng/ml (inset in c) leptin. (f) Adiponectin (10 mg/ml) abolishes the MDCK cell morphology induced by 1000 ng/ml leptin.

    Article Snippet: To examine the specificity of a leptin-induced effect on the morphology of MDCK cells, 25 mg/ml of rabbit polyclonal leptin antibody against rat leptin (BioVendor LLC, Candler, NC, USA) was added to the 20, 50, 100, or 1000 ng/ml leptin-treated cultures of MDCK cells alone.

    Techniques: Control, Concentration Assay

    Figure 9 | Adiponectin and leptin production of adipose tissue fragments (ATFs) in the absence or presence of Madin–Darby canine kidney (MDCK) cells in the supernatants of outer and inner dishes at 7 days in culture. In the cultures of ATFs alone, adiponectin and leptin are detected. Their concentration is not significantly different between the outer and inner dishes (P40.05). In the supernatants of outer dishes, adiponectin concentrations in the cocultures of ATFs and MDCK cells are significantly higher than those in the cultures of ATFs alone (Po0.05), whereas leptin concentrations are not significantly different between cultures of ATFs with and without MDCK cells (P40.05). In cocultures of ATFs and MDCK cells, adiponectin (Po0.01) and leptin (Po0.05) concentrations in the inner dishes were significantly lower than those in the outer dishes.

    Journal: Kidney international

    Article Title: Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture.

    doi: 10.1038/ki.2010.68

    Figure Lengend Snippet: Figure 9 | Adiponectin and leptin production of adipose tissue fragments (ATFs) in the absence or presence of Madin–Darby canine kidney (MDCK) cells in the supernatants of outer and inner dishes at 7 days in culture. In the cultures of ATFs alone, adiponectin and leptin are detected. Their concentration is not significantly different between the outer and inner dishes (P40.05). In the supernatants of outer dishes, adiponectin concentrations in the cocultures of ATFs and MDCK cells are significantly higher than those in the cultures of ATFs alone (Po0.05), whereas leptin concentrations are not significantly different between cultures of ATFs with and without MDCK cells (P40.05). In cocultures of ATFs and MDCK cells, adiponectin (Po0.01) and leptin (Po0.05) concentrations in the inner dishes were significantly lower than those in the outer dishes.

    Article Snippet: To examine the specificity of a leptin-induced effect on the morphology of MDCK cells, 25 mg/ml of rabbit polyclonal leptin antibody against rat leptin (BioVendor LLC, Candler, NC, USA) was added to the 20, 50, 100, or 1000 ng/ml leptin-treated cultures of MDCK cells alone.

    Techniques: Concentration Assay